Effect of rapamycin alone and in combination with sorafenib

Effect of rapamycin alone and in combination with sorafenib in an orthotopic model of human hepatocellular carcinoma.

 

OBJECTIVE
Novel therapeutic strategies are needed to prevent the tumor recurrence or metastasis after liver transplantation for hepatocellular carcinoma (HCC). This study was to investigate the effect of rapamycin, alone and in combination with sorafenib, on HCC in vivo.
METHODS
Xenograft of a highly metastatic human HCC tumor (LCI-D20) was used to evaluate primary tumor growth and lung metastasis after treatment with rapamycin alone or in combination with sorafenib.
Tumor cell proliferation was determined by Ki-67 immunostaining. To detect tumor cell apoptosis, the terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling assay was used. Tumor angiogenesis was investigated by using a monoclonal anti-CD31 antibody.
A vascular endothelial growth factor ELISA kit was used to measure vascular endothelial growth factor protein levels in the mice serum.
RESULTS
Rapamycin, alone and in combination with sorafenib, strongly inhibited primary tumor growth and lung metastases in LCI-D20 model.
Furthermore, the combination therapy significantly enhanced the effect of antitumor on primary tumor growth compared with single treatment with either rapamycin (P < 0.001) or sorafenib (P < 0.001).
Rapamycin alone inhibited HCC cell proliferation, induced apoptosis, and decreased tumor angiogenesis. Nevertheless, the combination therapy showed a significant inhibition of tumor cell proliferation (P < 0.05).
Additionally, the combination therapy also further enhanced suppression of tumor cell angiogenesis compared with rapamycin treatment (P < 0.01). However, the induction of apoptosis in combination therapy group was not significantly higher than in the rapamycin-treated group (P>> 0.05).
CONCLUSIONS
The combination therapy of rapamycin and sorafenib could be a new and promising therapeutic approach to the treatment of HCC and prevention of HCC recurrence after liver transplantation.
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SynBlock™ ELISA Blocking Buffer

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SynBlock Blocking Buffer-BB3

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Description: A competitive ELISA for quantitative measurement of Goat LMBR1 domain containing 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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Liver-related autoantibodies are crucial for the correct diagnosis and classification of autoimmune liver diseases (AiLD), namely autoimmune hepatitis types 1 and 2 (AIH-1 and 2), primary biliary cirrhosis (PBC), and the sclerosing cholangitis variants in adults and children.
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Sclerosing cholangitis presents as at least two variants, first the classical primary sclerosing cholangitis (PSC) mostly affecting adult men wherein the only (and non-specific) reactivity is an atypical perinuclear antineutrophil cytoplasmic antibody (p-ANCA), also termed perinuclear anti-neutrophil nuclear antibodies (p-ANNA) and second the childhood disease called autoimmune sclerosing cholangitis (ASC) with serological features resembling those of type 1 AIH.
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SARS-CoV-2 Neutralization Assay Development Kit (Delta Variant RBD-ACE2)

NTRL-DT-RBD-10 10 x 96-well plates
EUR 2390.76
Description: SARS-CoV-2 Neutralization Assay Development Kit (Delta Variant RBD-ACE2)

SARS-CoV-2 Neutralization Assay Development Kit (Kerala Variant RBD-ACE2)

NTRL-IN-RBD-10 10 x 96-well plates
EUR 2390.76
Description: SARS-CoV-2 Neutralization Assay Development Kit (Indian Variant RBD-ACE2)

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NTRL-LD-RBD-10 10 x 96-well plates
EUR 2390.76
Description: SARS-CoV-2 Neutralization Assay Development Kit (Lambda Variant RBD-ACE2)

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NTRL-BZ-RBD-10 10 x 96-well plates.
EUR 2390.76
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SARS-CoV-2 Neutralization Assay Development Kit (Omicron Variant SPIKE-ACE2)

NTRL-OMI-SPIKE-10 10 x 96-well plates
EUR 2390.76
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EUR 973.7
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EUR 2390.76
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DEIA533Cal 5 plates
EUR 1051.2
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9100 pack
EUR 649

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MBS258366-1Pack 1Pack
EUR 745

Antibody Sandwich ELISA Development Kit

MBS258366-5Packs 5Packs
EUR 3180

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MBS6006336-005mg 0.05(mg
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MBS6006336-5x005mg 5x0.05mg
EUR 2680

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abx370880-96tests 96 tests
EUR 1750

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EUR 1337.5

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MBS6011812-02mL 0.2(mL
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EUR 980

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EUR 980

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MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (FITC)

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MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (FITC)

MBS6320279-5x02mL 5x0.2mL
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MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (PE)

MBS6320286-02mL 0.2mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (PE)

MBS6320286-5x02mL 5x0.2mL
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MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 405)

MBS6320281-01mL 0.1mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 405)

MBS6320281-5x01mL 5x0.1mL
EUR 4250

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 490)

MBS6320282-01mL 0.1mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 490)

MBS6320282-5x01mL 5x0.1mL
EUR 4250

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 550)

MBS6320283-01mL 0.1mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 550)

MBS6320283-5x01mL 5x0.1mL
EUR 4250

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 650)

MBS6320284-01mL 0.1mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 650)

MBS6320284-5x01mL 5x0.1mL
EUR 4250

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 750)

MBS6320285-01mL 0.1mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (MaxLight 750)

MBS6320285-5x01mL 5x0.1mL
EUR 4250

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (Azide free) (HRP)

MBS6320280-02mL 0.2mL
EUR 980

MESDC2, CT (MESDC2, KIAA0081, MESD, LDLR chaperone MESD, Mesoderm development candidate 2, Mesoderm development protein, Renal carcinoma antigen NY-REN-61) (Azide free) (HRP)

MBS6320280-5x02mL 5x0.2mL
EUR 4250

Rat Embryonic Ectoderm Development ELISA kit

E02E0041-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Embryonic Ectoderm Development ELISA kit

E02E0041-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Embryonic Ectoderm Development ELISA kit

E02E0041-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Embryonic Ectoderm Development ELISA kit

E07E0041-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Embryonic Ectoderm Development ELISA kit

E07E0041-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Embryonic Ectoderm Development ELISA kit

E07E0041-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Embryonic Ectoderm Development ELISA kit

E08E0041-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Embryonic Ectoderm Development ELISA kit

E08E0041-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Embryonic Ectoderm Development ELISA kit

E08E0041-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Embryonic Ectoderm Development in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Embryonic Ectoderm Development ELISA kit

E01A12989 96T
EUR 700
Description: ELISA

Rat Embryonic Ectoderm Development ELISA Kit

MBS036617-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Embryonic Ectoderm Development ELISA Kit

MBS036617-48StripWells 48-Strip-Wells
EUR 550

Rat Embryonic Ectoderm Development ELISA Kit

MBS036617-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Embryonic Ectoderm Development ELISA Kit

MBS036617-96StripWells 96-Strip-Wells
EUR 765

Cat Embryonic Ectoderm Development ELISA Kit

MBS083691-INQUIRE INQUIRE Ask for price

Rat Embryonic Ectoderm Development ELISA Kit

MBS721185-10x96StripWells 10x96-Strip-Wells
EUR 5685

Rat Embryonic Ectoderm Development ELISA Kit

MBS721185-48StripWells 48-Strip-Wells
EUR 485

Rat Embryonic Ectoderm Development ELISA Kit

MBS721185-5x96StripWells 5x96-Strip-Wells
EUR 3020

Rat Embryonic Ectoderm Development ELISA Kit

MBS721185-96StripWells 96-Strip-Wells
EUR 690

Rat Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

abx534981-96tests 96 tests
EUR 687.5

Cat Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911642-INQUIRE INQUIRE Ask for price

Cavy Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911643-INQUIRE INQUIRE Ask for price

Duck Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911645-INQUIRE INQUIRE Ask for price

Fish Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911646-INQUIRE INQUIRE Ask for price

Goat Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911648-INQUIRE INQUIRE Ask for price

Rat Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911655-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) ELISA Kit

MBS9911655-48StripWells 48-Strip-Wells
EUR 550