Histidine-rich protein II: a novel technique to malaria drug sensitivity testing.
The manufacturing of histidine-rich protein II (HRP2), a histidine- and alanine-rich protein produced by Plasmodium falciparum, is intentlyassociated to the occasion and proliferation of the parasite and subsequently is utterly suited to mirror improvement inhibition as a measure of drug susceptibility.
It was the objective of the presentstudy to develop a malaria drug sensitivity assay primarily based on the measurement of HRP2 in a straightforward enzyme-linked immunosorbent assay (ELISA).
The model new verify proved to be as reliable as standard in vitro assays, whereas it was considerablyeasier to arrange and perform.
Parasites are incubated at an preliminary stage of parasitemia of 0.01 to 0.1% on microculture plates predosed with ascending concentrations of antimalarialdrugs.
After incubation for 48 to 72 h, the samples are freeze-thawed and transferred to ELISA plates. The completeELISA takes about 2.5 h to perform, is also carried out with commercially accessible verifykits, and requires comparatively little technical instruments.
In correlationanalysis, the outcomes intently paralleled these obtained by the isotopic assay (R = 0.892; P < 0.0001) and World Properly being Group schizont maturation exams (R = 0.959; P < 0.0001).
The novel HRP2 drug susceptibility assay proved to be very delicate, straightforwardto find out, and extraordinarilyreproducible.It might be used for a range of capabilities, from epidemiological analysis to the screening of latestdrugs,and can have the potential to interchange standard in vitro strategies.
Regular working procedures, updatedinformation, and analytical software program program might bediscovered from
Description: Description of target: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by KCNN2 is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. KCNN2 is a member of the KCNN family of potassium channel genes.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.059ng/mL
Description: Description of target: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene is a member of the KCNN family of potassium channel genes. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. Alternate splicing of this gene results in multiple transcript variants.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.081 ng/mL
Description: A polyclonal antibody against KCNN2. Recognizes KCNN2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB.IHC, ELISA;WB:1:1000-2000, IHC-p:1:100-200
Description: Additional name(s) for this target protein: Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 antibody, KCa2.2
Description: A polyclonal antibody for detection of KCNN2 SK2) from Human, Mouse, Rat. This KCNN2 SK2) antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide
Description: A polyclonal antibody for detection of KCNN2 SK2) from Human, Mouse, Rat. This KCNN2 SK2) antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide
Description: A polyclonal antibody for detection of KCNN2 SK2) from Human, Mouse, Rat. This KCNN2 SK2) antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthetic peptide
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human KCNN2 (Internal). This antibody is tested and proven to work in the following applications:
Description: A sandwich quantitative ELISA assay kit for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in Tissue homogenates and other biological fluids.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in Tissue homogenates and other biological fluids.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in Tissue homogenates and other biological fluids.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in Tissue homogenates and other biological fluids.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2)ELISA Kit
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KCNN2 (C-Terminus). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KCNN2 - middle region. This antibody is tested and proven to work in the following applications:
KCNN2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
Description: A sandwich ELISA kit for detection of Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2)
Description: Quantitative sandwich ELISA for measuring Human Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2)
Description: Quantitative sandwich ELISA for measuring Human Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KCNN2 - C-terminal region. This antibody is tested and proven to work in the following applications:
Prognostic significance of plasma vascular endothelial improvementsituation ranges in victims with hormone-refractory prostate most cancers dealt with on Most cancers and Leukemia Group B 9480.
OBJECTIVE
Plasma vascular endothelialimprovementsituation (VEGF) ranges are significantly elevated in victims with hormone-refractory prostate most cancers (HRPC) in distinction with victims with localized sickness and have been associated to sicknessimprovement in totally different most cancers affected particular personpopulations.
Subsequently, we measured VEGF ranges in plasma prospectively collected from victims enrolled in Most cancers and Leukemia Group B 9480, an intergroupstudy of suramin in victims with HRPC, to search out out whether or not or not these ranges had prognostic significance.
METHODS
Pretreatment plasma was collected from victims with HRPC enrolled in Most cancers and Leukemia Group B 9480. In a subset of samples advisor of the entire cohort, plasma VEGF ranges have been determined in duplicate using a Quantiglo chemiluminescentELISAgear (R&D Methods, Minneapolis, MN).
Statistical analyses have been carried out to search out out the correlation between pretreatment plasma VEGF ranges and time of common survival. The proportional hazards model was used to judge the prognostic significance of assorteddecreasecomponents in multivariate fashions.
RESULTS
Plasma VEGF ranges on this inhabitants ranged from 4-885 pg/ml, with a median stage of 83 pg/ml. As a regular variable, plasma VEGF ranges inversely correlated with survival time (P = 0.002).
Usingquite a fewexploratorydecreasecomponents, plasma VEGF ranges appeared to correlate with survival. In multivariate fashions wherebytotally different prognostic components (serum prostate-specific antigen, alkaline phosphatase, proof of measurable sickness, and hemoglobin) have been included, plasma VEGF ranges have been very important at quite a fewdecreasecomponents examined.
CONCLUSIONS
Although these information are exploratory and ought to be confirmed in an neutralinformation set, they counsel that VEGF may have medical significance in victims with HRPC.
Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry.
Practiceprovides many nicely being benefits, along with improved metabolism, cardiovascular nicely being, and cognition. Now we’veconfirmed beforehand that FNDC5, a sort I transmembrane protein, and its circulating type, irisin, convey just a fewof thosebenefits in mice.
However, presentresearch questioned the existence of circulating human irisin everybecause of human FNDC5 has a non-canonical ATA translationstart and because ofclaims that many human irisin antibodies utilized in industrial ELISAkits lack required specificity.
On this paperwe haveacknowledged and quantitated human irisin in plasmausing mass spectrometry with administration peptides enriched with heavy safe isotopes as insidenecessities.
This actual state-of-the-art methodology reveals that human irisin is verytranslated from its non-canonical startcodon and circulates at ∼ 3.6 ng/ml in sedentary folks; this stage is elevated to ∼ 4.Three ng/ml in folkscurrentcourse of cardio interval teaching.
These informationunequivocallypresent that human irisin exists, circulates, and is regulated by prepare.
Human angiopoietin receptor Tie1,ANG-R-Tie1 ELISA kit
Description: A sandwich ELISA kit for quantitative measurement of Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) in samples from Serum, Plasma, Cell supernatant
Description: Soluble TIE-1 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a homodimeric, glycosylated, polypeptide containing 749 amino acids and having a total molecular mass of 250 kDa. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.;The TIE1 Fc Chimera is purified by proprietary chromatographic techniques.
Description: A sandwich ELISA kit for quantitative measurement of Mouse ANG-R-Tie1 (Angiopoietin Receptor Tie1) in samples from Serum, Plasma, Cell supernatant
Description: A sandwich CLIA kit for quantitative measurement of Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) in samples from Serum, Plasma, Cell supernatant
Description: A polyclonal antibody against TIE1. Recognizes TIE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000
Description: A polyclonal antibody against TIE1. Recognizes TIE1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against TIE1. Recognizes TIE1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against TIE1. Recognizes TIE1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
Description: A polyclonal antibody against TIE1. Recognizes TIE1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB
Description: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Description: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Description: Soluble TIE-1 Mouse Recombinant fused with the Fc part of human IgG1 produced in CHO cells is a glycosylated disulfide-linked homodimeric polypeptide chain containing amino acids 23-749 and having a total molecular mass of 260kDa. The Mouse TIE-1/Fc monomer has a calculated molecular mass of approximately 105kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130kDa protein in SDS-PAGE under reducing conditions.;The TIE1 Fc Chimera is purified by proprietary chromatographic techniques.
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