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Comparison of multiplex immunoassay platforms.
Andres- posted on
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Comparability of multiplex immunoassay platforms.
BACKGROUND
Candidate biomarkers found with high-throughput proteomic methods (together with many biomarkers reported within the literature) have to be rigorously validated.
The simultaneous quantitative evaluation of a number of potential biomarkers throughout giant cohorts presents a serious problem to the sector.
Multiplex immunoassays symbolize a promising answer, with the potential to offer quantitative knowledge through parallel analyses. These assays additionally require considerably much less pattern and reagents than the normal ELISA (which is additional restricted by its capacity to measure solely a single antigen).
We have now measured the reproducibility, reliability, robustness, accuracy, and throughput of commercially accessible multiplex immunoassays to determine their suitability for serum biomarker evaluation and validation.
METHODS
Assay platforms MULTI-ARRAY (Meso Scale Discovery), Bio-Plex (Bio-Rad Laboratories), A(2) (Beckman Coulter), FAST Quant (Whatman Schleicher & Schuell BioScience), and FlowCytomix (Bender MedSystems) have been chosen as consultant examples of applied sciences at present used for high-throughput immunoanalysis.
All assays have been carried out in response to protocols specified by the producers and with the reagents (diluents, calibrators, blocking reagents, and detecting-antibody mixtures) included with their kits.
RESULTS
The quantifiable interval decided for every assay and antigen was based mostly on precision (CV < 25%) and proportion restoration (measured focus inside 20% of the particular focus).
The MULTI-ARRAY and Bio-Plex assays had the perfect efficiency with the lowest limits of detection, and the MULTI-ARRAY system had essentially the most linear sign output over the widest focus vary (10(5) to 10(6)).
Cytokine concentrations in unspiked and cytokine-spiked serum samples from wholesome people have been additional investigated with the MULTI-ARRAY and Bio-Plex assays.
CONCLUSIONS
The MULTI-ARRAY and Bio-Plex multiplex immunoassay techniques are essentially the most appropriate for biomarker evaluation or quantification.
 Block Wash Buffer |
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| I044 | Cygnus Technologies | 1000 ml | EUR 527 |
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Description: Block Wash Buffer by Cygnus Technologies is available in Europe via Gentaur. |
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 10X Loading Buffer |
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| L0503-005 | GenDepot | 5X1ml | EUR 77 |
10X Loading Buffer |
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| L0504-005 | GenDepot | 5X1ml | EUR 77 |
 10X MOPS Buffer |
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| M1057-050 | GenDepot | 500ml | EUR 161 |
10X MOPS Buffer |
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| M1057-100 | GenDepot | 2X500ml | EUR 219 |
 NH4 Buffer, 10x |
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| BIO-37025 | Bioline | 3 x 1.2ml | Ask for price |
 10x Taq Buffer |
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| BA00151 | Bioatlas | 1.8ml | EUR 55 |
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Description: High purity buffer for various PCR applications. |
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 10X TAE Buffer |
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| T8048-101 | GenDepot | 1L | EUR 89 |
 TBS Buffer, 10X |
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| T8057-100 | GenDepot | 1L | EUR 115 |
TBS Buffer, 10X |
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| T8057-105 | GenDepot | 5x1L | EUR 286 |
) Wash Buffer (20X Concentrate) |
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| 85R-126 | Fitzgerald | 250 ml | EUR 197 |
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Description: 10X concentrate ELISA wash buffer, ready to use |
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 Wash Buffer Concentrate, 125ML |
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| X007-125ML | Arbor Assays | 125ML | EUR 194 |
 Wash Buffer Concentrate, 30ML |
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| X007-30ML | Arbor Assays | 30ML | EUR 122 |
Wash Buffer Concentrate, 30ML |
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| X135-30ML | Arbor Assays | 30ML | EUR 122 |
 TBS Wash Buffer Pack |
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| AR0144 | BosterBio | 500mL/pack | EUR 60 |
 PBS Wash Buffer Pack |
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| AR1155 | BosterBio | 500mL/pack | EUR 65 |
 Inclusion Body Wash Buffer |
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| PL032-10ml | Bio Basic | 10mL | EUR 58.7 |
Inclusion Body Wash Buffer |
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| PL032-5x10ml | Bio Basic | 5x10ml, 50ml | EUR 84.8 |
 AlbuVoid Wash Buffer AVWB |
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| AVWB-1000 | Biotech Support Group | 1000 mL | EUR 456 |
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Description: Albuvoid™ Buffer |
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AlbuVoid Wash Buffer AVWB |
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| AVWB-500 | Biotech Support Group | 500 mL | EUR 245 |
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Description: Albuvoid™ Buffer |
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 Michel's Transport Wash Buffer |
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| MTW-10000 | ScyTek Laboratories | 10 L | EUR 706 |
Michel's Transport Wash Buffer |
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| MTW-20000 | ScyTek Laboratories | 20 L | EUR 1297 |
Michel's Transport Wash Buffer |
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| MTW500 | ScyTek Laboratories | 500 ml | EUR 98 |
Michel's Transport Wash Buffer |
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| MTW999 | ScyTek Laboratories | 1000 ml | EUR 127 |
 HemoVoid Wash Buffer HVWB |
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| HVWB-1000 | Biotech Support Group | 1000 mL | EUR 456 |
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Description: HemoVoid™ Buffer |
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HemoVoid Wash Buffer HVWB |
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| HVWB-500 | Biotech Support Group | 500 mL | EUR 245 |
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Description: HemoVoid™ Buffer |
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 for ELISA) Wash buffer concentrate (20X) for ELISA |
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| 80080 | Alpha Diagnostics | 100 ml | EUR 164 |
 for ELISA) Wash buffer concentrate (100X) for ELISA |
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| WB-150 | Alpha Diagnostics | 50 ml | EUR 116 |
) Binding/Coating Buffer (10X) |
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| 85R-124 | Fitzgerald | 250 ml | EUR 216 |
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Description: ELISA buffer for optimal coating and binding of antibodies and antigens |
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 10X KRB-IBMX Buffer |
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| 122937 | Mediomics | 1 Vial | EUR 77 |
 Blocking Buffer, 10X, 250ML |
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| X109-250ML | Arbor Assays | 250ML | EUR 359 |
 Blocking Buffer, 10X, 25ML |
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| X109-25ML | Arbor Assays | 25ML | EUR 122 |
 Alkaline Phosphate Buffer, 10x |
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| K2191050-6 | Biochain | 50 ml | EUR 137 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 10X TAE buffer solution |
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| A00239 | Bio Basic | 500ml | EUR 56.36 |
 25ML 10X CE Buffer |
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| NAT1206 | National Diagnostics | 25ML | EUR 115 |
 100ML 10X CE Buffer |
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| NAT1208 | National Diagnostics | 100ML | EUR 164 |
 10x TAE Buffer Solution |
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| BA01801 | Bioatlas | 6x100ml | EUR 97 |
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Description: High purity buffer for various PCR applications. |
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 10x PBS Buffer Solution |
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| BA01802 | Bioatlas | 6x100ml | EUR 97 |
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Description: High purity buffer for various PCR applications. |
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 10x TBE Buffer Solution |
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| BA01804 | Bioatlas | 6x100ml | EUR 97 |
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Description: High purity buffer for various PCR applications. |
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) 10x Taq Buffer (MgCl2) |
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| PCRB16 | Bio Basic | 4x1.5ml, 6ml | EUR 58.7 |
 10x Gel Loading Buffer |
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| TG4038 | TopoGen | 1ml | EUR 156 |
 ToxOut? Rapid Removal Wash Buffer |
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| M7947-50 | Biovision | EUR 185 | |
 UPCK Wash Buffer UPWB™ |
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| UPWB-1000 | Biotech Support Group | 1000 mL | EUR 456 |
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Description: UPCK™ Buffer |
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UPCK Wash Buffer UPWB™ |
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| UPWB-500 | Biotech Support Group | 500 mL | EUR 245 |
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Description: UPCK™ Buffer |
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) 10X Tris-Glycine Native Buffer (Transfer buffer) |
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| T8052-050 | GenDepot | 500ml | EUR 80 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
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| T8052-100 | GenDepot | 2X500ml | EUR 104 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
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| T8052-101 | GenDepot | 1L | EUR 95 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
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| T8052-200 | GenDepot | 4X500ml | EUR 128 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
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| T8052-201 | GenDepot | 2X1L | EUR 128 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
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| T8052-401 | GenDepot | 4X1L | EUR 165 |
 10X Annexin V Binding Buffer |
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| 1006-100 | Biovision | EUR 196 | |
 1 L TAE BUFFER, 10X |
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| 46-010-CM | CORNING | 1L/pk | EUR 105 |
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Description: Media Catalog; Classical Media |
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 Aniline, 10X in acetate buffer |
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| 91057 | Biotium | 10ML | EUR 111 |
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Description: Minimum order quantity: 1 unit of 10ML |
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 10X PBS Buffer , pH 7.4 |
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| P2100-050 | GenDepot | 500ml | EUR 88 |
10X PBS Buffer , pH 7.4 |
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| P2100-100 | GenDepot | 1L | EUR 91 |
) Xpert Native Transfer Buffer(10X) |
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| P9001-050 | GenDepot | 500ml | EUR 93 |
Xpert Native Transfer Buffer(10X) |
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| P9001-100 | GenDepot | 1L | EUR 138 |
 10x Tris-Glycine Buffer Solution |
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| BA01806 | Bioatlas | 6x100ml | EUR 97 |
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Description: High purity buffer for various PCR applications. |
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 PBST buffer 10x Strength Solution |
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| PW004 | Bio Basic | 500ml | EUR 67.4 |
 TBST buffer 10x Strength Solution |
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| PW009 | Bio Basic | 500ml | EUR 65.66 |
) Annexin V Binding Buffer (10x) |
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| EXB0019 | ExBio | N/A | EUR 86 |
2SO4) 10x Taq Buffer (NH4)2SO4 |
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| PCRB55 | Bio Basic | 4x1.5ml, 6ml | EUR 58.7 |
 SuperBlot Western Transfer buffer 10X |
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| DG-WEST | Bio Basic | 500ml | EUR 113.8 |
 10X Tris-Glycine SDS buffer |
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| T8053-050 | GenDepot | 500ml | EUR 85 |
10X Tris-Glycine SDS buffer |
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| T8053-100 | GenDepot | 2X500ml | EUR 111 |
10X Tris-Glycine SDS buffer |
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| T8053-101 | GenDepot | 1L | EUR 99 |
10X Tris-Glycine SDS buffer |
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| T8053-104 | GenDepot | 4 L | EUR 153 |
10X Tris-Glycine SDS buffer |
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| T8053-200 | GenDepot | 4X500ml | EUR 136 |
10X Tris-Glycine SDS buffer |
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| T8053-201 | GenDepot | 2X1L | EUR 135 |
10X Tris-Glycine SDS buffer |
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| T8053-401 | GenDepot | 4X1L | EUR 172 |
 TBS Buffer, 10X , Refill Pack |
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| T8057-401 | GenDepot | 1 Gal | EUR 182 |
 Topo I Assay Buffer 10x |
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| TG4010 | TopoGen | 1ml | EUR 145 |
) West-Ezier Washing Buffer(10X) |
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| W3873-050 | GenDepot | 500ml | EUR 118 |
 pH 6.0) Citrate Buffer (10x) pH 6.0 |
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| CBB125 | ScyTek Laboratories | 125 ml | EUR 66 |
Citrate Buffer (10x) pH 6.0 |
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| CBB500 | ScyTek Laboratories | 500 ml | EUR 80 |
Citrate Buffer (10x) pH 6.0 |
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| CBB999 | ScyTek Laboratories | 1000 ml | EUR 98 |
) Colloidal Gold Running Buffer (10X) |
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| CG-RB10 | Alpha Diagnostics | 10 ml | EUR 225 |
; ELISPOT) Dilution buffer R (10x); ELISPOT |
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| CT348 | U-CyTech | 10 ml | EUR 78 |
; ELISPOT) Dilution buffer T (10x); ELISPOT |
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| CT358 | U-CyTech | 8 ml | EUR 78 |
 10x PCR Buffer without MgCl2 |
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| ABP-PP-ERBF001 | Allele Biotech | 1x1ml | Ask for price |
10x PCR Buffer without MgCl2 |
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| ABP-PP-ERBF002 | Allele Biotech | 2x1ml | Ask for price |
10x PCR Buffer without MgCl2 |
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| ABP-PP-ERBF005 | Allele Biotech | 5x1ml | Ask for price |
 10x PCR Buffer with MgCl2 |
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| ABP-PP-ERBFM01 | Allele Biotech | 1x1ml | Ask for price |
10x PCR Buffer with MgCl2 |
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| ABP-PP-ERBFM02 | Allele Biotech | 2x1ml | Ask for price |
10x PCR Buffer with MgCl2 |
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| ABP-PP-ERBFM05 | Allele Biotech | 5x1ml | Ask for price |
 for ELISA, 10 ml) Wash buffer concentrate (100X) for ELISA, 10 ml |
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| WB-100 | Alpha Diagnostics | 10 ml | EUR 103 |
 10X Red Blood Cell Lysis Buffer |
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| 5831-100 | Biovision | EUR 120 | |
 10X Solution) TG Buffer (TRIS-GLYCINE) 10X Solution |
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| A0028 | Bio Basic | 4L | EUR 94.8 |
 1 L TBE BUFFER, 10X, LIQUID |
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| 46-011-CM | CORNING | 1L/pk | EUR 118 |
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Description: Media Catalog; Classical Media |
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) Finegel SDS-PAGE Running Buffer(10X) |
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| F1504-050 | GenDepot | 500ml | EUR 151 |
Finegel SDS-PAGE Running Buffer(10X) |
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| F1504-100 | GenDepot | 2x500ml | EUR 201 |
 1L Tris-Glycine Electroblotting Buffer 10X |
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| NAT1240 | National Diagnostics | 1L | EUR 85 |
 4L Tris-Glycine Electroblotting Buffer 10X |
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| NAT1242 | National Diagnostics | 4L | EUR 121 |
No affiliation of CSF biomarkers with APOEepsilon4, plaque and tangle burden in particular Alzheimer’s illness.
he CSF biomarkers beta-amyloid peptide (Abeta(1-42)), whole tau protein (T-tau) and tau phosphorylated at threonine 181 (P-tau(181P)) have been decided in autopsy-confirmed Alzheimer’s illness sufferers to be able to examine doable associations with the epsilon4 allele of APOE and density and unfold of plaques (SP) and tangles (NFT).
CSF ranges of Abeta(1-42), T-tau and P-tau(181P) have been decided in 50 Alzheimer’s illness sufferers utilizing commercially accessible single parameter ELISA kits (INNOTEST(R)).
Genomic DNA was extracted from complete blood and the APOE genotype was decided utilizing normal strategies. Tangle burden was assessed by way of Braak’s NFT phases (I-VI), whereas the plaque burden was assessed by way of Braak’s SP phases (A-C).
CSF biomarker ranges weren’t totally different when evaluating epsilon4 carriers (n = 21) and non-carriers (n = 29) (P>> 0.05 for all comparisons). No vital correlations between the variety of epsilon4 alleles (0, 1 or 2) and CSF ranges of Abeta(1-42) (Spearman Rank Order: r = -0.057, P = 0.695), T-tau (r = 0.104, P = 0.472) and P-tau(181P) (r = 0.062, P = 0.668) have been discovered. Braak’s SP (Abeta(1-42): r = -0.155, P = 0.280; T-tau: r = -0.044, P = 0.763; P-tau(181P): r = -0.010, P = 0.947) and NFT (Abeta(1-42): r = -0.145, P = 0.315; T-tau: r = 0.117, P = 0.415; P-tau(181P): r = 0.150, P = 0.296) phases weren’t considerably correlated with CSF biomarker ranges. In conclusion, CSF ranges of Abeta(1-42), T-tau and P-tau(181P) weren’t related with epsilon4, tangle or plaque burden in 50 autopsy-confirmed Alzheimer’s illness sufferers.
Within the mild of future biomarker functions like monitoring of illness development and as allocortical neuropathological modifications considerably contribute to scientific signs, the idea of in vivo surrogate biomarkers ought to be additional explored.
Analysis of commercially accessible anti-dengue virus immunoglobulin M checks.
-dengue virus immunoglobulin M kits have been evaluated.
Take a look at sensitivities have been 21%-99% and specificities have been 77%-98% in contrast with reference ELISAs. False-positive outcomes have been discovered for sufferers with malaria or previous dengue infections.
Three ELISAs exhibiting robust settlement with reference ELISAs will likely be included within the World Well being Group Bulk Procurement Scheme.
, Sterile-Filtered) StemBoost? 2i-Reprogramming Cocktail Set (1000X), Sterile-Filtered |
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| K889-set | Biovision | EUR 620 | |
, Sterile-Filtered) StemBoost? SMAD Signaling Inhibitor Cocktail Set (1000X), Sterile-Filtered |
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| K877-set | Biovision | EUR 512 | |
, Sterile-Filtered) StemBoost? Neuronal Cell Induction Cocktail Set (100X), Sterile-Filtered |
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| K891-set | Biovision | EUR 805 | |
 Antigen-Antibody Pens, a set of any 3 pens |
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| PEN13-SET | Alpha Diagnostics | 3 | EUR 451 |
 Tool for opening/closing PCR strips, pk/2 |
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| P0800-TL | MTC Bio | 2/pack | EUR 108.05 |
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Description: Tool for opening/closing PCR strips |
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, IgM, and IgE isotype controls (set of 7 IgGs)) Mouse IgA, IgGs (1, 2a, 2b, 3), IgM, and IgE isotype controls (set of 7 IgGs) |
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| 20102-SET | Alpha Diagnostics | 1 Set (100 ugx7) | EUR 598 |
 bottle set (3 bottles and cap assembly)) ELISA Microplate Washer Automated (#MPW-30) bottle set (3 bottles and cap assembly) |
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| MPW-30-SET | Alpha Diagnostics | 1 | EUR 408 |
 FURA-4F/AM |
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| 9550-set | Biovision | EUR 349 | |
 FURA-5F/AM |
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| 9551-set | Biovision | EUR 398 | |
 Conversion Cocktail, ZAPK) StemBoost™ iN (induced Neuron) Conversion Cocktail, ZAPK |
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| S250-set | Biovision | 5 x 1 ml | EUR 784 |
 FURA-2 Am |
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| 2243-set | Biovision | EUR 153 | |
 Sulfo-SMCC |
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| 2320-set | Biovision | EUR 229 | |
 Sulfo-NHS |
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| 2321-set | Biovision | EUR 202 | |
 Sulfo-NHS-Biotin |
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| 2322-set | Biovision | EUR 185 | |
 Sulfo-NHS-SS-Biotin |
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| 2323-set | Biovision | EUR 229 | |
 Sulfo-NHS-LC-Biotin |
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| 2326-set | Biovision | EUR 213 | |
 BS3 Crosslinker |
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| 2327-set | Biovision | EUR 207 | |
 Triton® X-100, MegaPure? Detergent, 10% Solution |
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| 2124-set | Biovision | EUR 175 | |
 Tween® 20, MegaPure? Detergent, 10% Solution |
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| 2125-set | Biovision | EUR 185 | |
 Tween® 80, MegaPure? Detergent, 10% Solution |
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| 2126-set | Biovision | EUR 191 | |
 NP-40 Substitute, MegaPure? Detergent, 10% Solution |
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| 2127-set | Biovision | EUR 185 | |
 Triton® X-114, MegaPure? Detergent, 10% Solution, Sterile-Filtered |
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| 2137-set | Biovision | EUR 175 | |
 IGEPAL® CA-630 MegaPure? Detergent,10% Solution |
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| B1831-set | Biovision | EUR 240 | |
 EZSolution? D-Luciferin, Potassium Salt |
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| B3001-set | Biovision | EUR 370 | |
) EZCell? Cell Stimulation Cocktail (500X) |
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| K930-set | Biovision | EUR 305 | |
) EZCell? Cell Stimulation and Protein Transport Inhibitor Cocktail (500X) |
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| K931-set | Biovision | EUR 370 | |
 StemBoost? Reprogramming Cocktail, SMC4 |
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| S231-set | Biovision | EUR 838 | |
 Cocktail) StemBoost? Fate Maintenance Medium (FMM) Cocktail |
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| S232-set | Biovision | EUR 827 | |
 StemBoost? Reprogramming Cocktail, SLCD |
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| S245-set | Biovision | EUR 838 | |
) StemBoost? Reprogramming Cocktail, VCRFBI, Sterile-Filtered (100X) |
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| S246-set | Biovision | EUR 838 | |
, Sterile-Filtered) StemBoost? Reprogramming Cocktail, 3i (1000X), Sterile-Filtered |
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| S247-set | Biovision | EUR 620 | |
) EZBlock? Phosphatase Inhibitor Cocktail I (50X) |
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| K273-set | Biovision | EUR 381 | |
 EZBlock? Phosphatase Inhibitor Cocktail II |
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| K275-set | Biovision | EUR 300 | |
 EZBlock? Phosphatase Inhibitor Cocktail III |
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| K276-set | Biovision | EUR 365 | |
 EZBlock? Protease Inhibitor Cocktail II |
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| K277-set | Biovision | EUR 234 | |
 EZBlock? Protease Inhibitor Cocktail III |
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| K278-set | Biovision | EUR 240 | |
 EZBlock? Protease Inhibitor Cocktail XII, EDTA-free, Animal-free |
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| K221-set | Biovision | EUR 283 | |
 EZBlock? Protease Inhibitor Cocktail XIII, EDTA & Animal-Free |
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| K222-set | Biovision | EUR 332 | |
 EZBlock? Protease Inhibitor Cocktail, EDTA-Free |
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| K272-set | Biovision | EUR 294 | |
 EZBlock? Protease Inhibitor Cocktail IV |
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| K279-set | Biovision | EUR 262 | |
 EZBlock? Phosphatase Inhibitor Cocktail IV |
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| K282-set | Biovision | EUR 490 | |
 EZBlock? Universal Protease and Phosphatase Inhibitor Cocktail |
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| K283-set | Biovision | EUR 620 | |
 EZBlock? Universal Protease and Phosphatase Inhibitor Cocktail , EDTA-Free |
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| K284-set | Biovision | EUR 626 | |
 EZBlock? Protease Inhibitor Cocktail V, EDTA-Free |
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| K290-set | Biovision | EUR 224 | |
 EZBlock? Protease Inhibitor Cocktail VI, General Use |
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| K291-set | Biovision | EUR 229 | |
 EZBlock? Protease Inhibitor Cocktail VII |
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| K292-set | Biovision | EUR 392 | |
 EZBlock? Protease Inhibitor Cocktail VIII |
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| K293-set | Biovision | EUR 392 | |
 EZBlock? Protease Inhibitor Cocktail IX |
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| K294-set | Biovision | EUR 392 | |
 EZBlock? Protease Inhibitor Cocktail X, EDTA-Free |
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| K297-set | Biovision | EUR 234 | |
 EZBlock? Phosphatase Inhibitor Cocktail XI |
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| K298-set | Biovision | EUR 294 | |
 EZBlock? Protease Inhibitor Cocktail XV |
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| K393-set | Biovision | EUR 479 | |
 EZBlock? Protease Inhibitor Cocktail II, Animal-Free |
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| K394-set | Biovision | EUR 294 | |
 EZBlock? Protease Inhibitor Cocktail XIV |
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| K223-set | Biovision | EUR 343 | |
 EZBlock? Protease Inhibitor Cocktail VIII, DMSO-Free |
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| K395-set | Biovision | EUR 392 | |
 Set/ Rat Set ELISA Kit |
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| ELI-35832r | Lifescience Market | 96 Tests | EUR 886 |
 Set ELISA Kit| Rat Protein SET ELISA Kit |
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| EF019316 | Lifescience Market | 96 Tests | EUR 689 |
 Set ELISA Kit| Mouse Protein SET ELISA Kit |
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| EF016190 | Lifescience Market | 96 Tests | EUR 689 |
 Human Protein SET, SET ELISA KIT |
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| ELI-29726h | Lifescience Market | 96 Tests | EUR 824 |
 Mouse Protein SET, Set ELISA KIT |
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| ELI-52934m | Lifescience Market | 96 Tests | EUR 865 |
 ELISA Kit) Mouse Protein SET (SET) ELISA Kit |
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| abx556068-96tests | Abbexa | 96 tests | EUR 668 |
 ELISA Kit) Rat Protein SET (SET) ELISA Kit |
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| abx556216-96tests | Abbexa | 96 tests | EUR 668 |
 ELISA Kit) Human Protein SET (SET) ELISA Kit |
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| abx555962-96tests | Abbexa | 96 tests | EUR 668 |
 Human SET/ Protein SET ELISA Kit |
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| E2270Hu | Sunlong | 1 Kit | EUR 605 |
 SET ELISA KIT|Human |
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| EF002839 | Lifescience Market | 96 Tests | EUR 689 |
 (OKEH06547)) SET ELISA Kit (Human) (OKEH06547) |
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| OKEH06547 | Aviva Systems Biology | 96 Wells | EUR 662 |
|
Description: Description of target: Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.176 ng/mL |
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 Antibody) Protein SET (SET) Antibody |
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| 20-abx338613 | Abbexa |
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Protein SET (SET) Antibody |
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| abx430042-200ul | Abbexa | 200 ul | EUR 384 |
 Protein) Protein SET (SET) Protein |
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| 20-abx261106 | Abbexa |
|
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Protein SET (SET) Antibody |
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| abx237764-100ug | Abbexa | 100 ug | EUR 509 |
Protein SET (SET) Antibody |
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| 20-abx004747 | Abbexa |
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Protein SET (SET) Antibody |
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| 20-abx141819 | Abbexa |
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Protein SET (SET) Antibody |
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| abx028158-400ul | Abbexa | 400 ul | EUR 523 |
Protein SET (SET) Antibody |
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| abx028158-80l | Abbexa | 80 µl | EUR 286 |
 ELISA kit for Human Protein SET |
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| EK3370 | SAB | 96 tests | EUR 553 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Human Protein SET in samples from serum, plasma, tissue homogenates and other biological fluids. |
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 Antibody (HRP)) Protein SET (SET) Antibody (HRP) |
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| 20-abx337312 | Abbexa |
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 Antibody (FITC)) Protein SET (SET) Antibody (FITC) |
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| 20-abx337313 | Abbexa |
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 Antibody (Biotin)) Protein SET (SET) Antibody (Biotin) |
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| 20-abx337314 | Abbexa |
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 Human SET- binding protein, SETBP1 ELISA KIT |
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| ELI-13557h | Lifescience Market | 96 Tests | EUR 824 |
 Mouse SET- binding protein, Setbp1 ELISA KIT |
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| ELI-39341m | Lifescience Market | 96 Tests | EUR 865 |
 Human CD200 Molecule, CD200 ELISA Pair Set |
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| DEIA234 | Creative Diagnostics | 5 plates | EUR 1368 |
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Description: The Human CD200 ELISA Pair Set is for the quantitative determination of Human CD200. |
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) Set sgRNA CRISPR Lentivector set (Rat) |
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| K6753701 | ABM | 3 x 1.0 ug | EUR 339 |
) Set sgRNA CRISPR Lentivector set (Mouse) |
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| K4424601 | ABM | 3 x 1.0 ug | EUR 339 |
) SET sgRNA CRISPR Lentivector set (Human) |
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| K2127601 | ABM | 3 x 1.0 ug | EUR 339 |
 SET antibody |
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| 70R-5618 | Fitzgerald | 50 ug | EUR 467 |
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Description: Rabbit polyclonal SET antibody raised against the N terminal of SET |
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 dNTP Set |
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| G050 | ABM | 4 x 250 ul (100 mM each) | EUR 111 |
 NTP Set |
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| G946 | ABM | 4 x 250 ul (100 mM each) | EUR 139 |
SET antibody |
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| 70R-12995 | Fitzgerald | 100 ul | EUR 457 |
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Description: Affinity purified Rabbit polyclonal SET antibody |
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SET antibody |
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| 70R-20188 | Fitzgerald | 50 ul | EUR 435 |
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Description: Rabbit polyclonal SET antibody |
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 SET Antibody |
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| 1-CSB-PA021099GA01HU | Cusabio |
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Description: A polyclonal antibody against SET. Recognizes SET from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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SET Antibody |
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| 1-CSB-PA875345LA01HU | Cusabio |
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Description: A polyclonal antibody against SET. Recognizes SET from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000 |
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